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Arraystar inc circrna array v 2.0
Primer sequences used for qRT-PCR analysis of <t> circRNA </t> and mRNA levels.
Circrna Array V 2.0, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/circrna array v 2.0/product/Arraystar inc
Average 90 stars, based on 1 article reviews
circrna array v 2.0 - by Bioz Stars, 2026-06
90/100 stars

Images

1) Product Images from "Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention"

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

Journal: Frontiers in Medicine

doi: 10.3389/fmed.2021.626948

Primer sequences used for qRT-PCR analysis of  circRNA  and mRNA levels.
Figure Legend Snippet: Primer sequences used for qRT-PCR analysis of circRNA and mRNA levels.

Techniques Used:

CircRNAs comparing the IRI and sham groups and between the IPC and IRI groups. The left (scatterplots) and right (volcano plots) show the alteration of circRNA expression between IRI and sham groups (A) and between IPC and IRI groups (B) . In the scatterplot, the values on the X - and Y -axes are the log2 scaled signals of samples. Fold change is represented by the green lines. The circRNAs outside the range formed by the upper and lower green lines are those with the fold change ≥ 1.5 between the compared groups. In the volcano plot, the vertical green lines represent a 1.5-fold change although the horizontal green line corresponds to a P -value of 0.05. The red points in the volcano plot represent the significantly altered circRNAs with P -value <0.05. (C) Different subgroups of significantly altered circRNAs according to their effects and position. (D) Heat maps of circRNA profiles from the microarray data. The color scales represent expression values. Red represents high expression, and green indicates low relative expression. Each row of colored boxes indicates a single circRNA, each column indicates a single sample.
Figure Legend Snippet: CircRNAs comparing the IRI and sham groups and between the IPC and IRI groups. The left (scatterplots) and right (volcano plots) show the alteration of circRNA expression between IRI and sham groups (A) and between IPC and IRI groups (B) . In the scatterplot, the values on the X - and Y -axes are the log2 scaled signals of samples. Fold change is represented by the green lines. The circRNAs outside the range formed by the upper and lower green lines are those with the fold change ≥ 1.5 between the compared groups. In the volcano plot, the vertical green lines represent a 1.5-fold change although the horizontal green line corresponds to a P -value of 0.05. The red points in the volcano plot represent the significantly altered circRNAs with P -value <0.05. (C) Different subgroups of significantly altered circRNAs according to their effects and position. (D) Heat maps of circRNA profiles from the microarray data. The color scales represent expression values. Red represents high expression, and green indicates low relative expression. Each row of colored boxes indicates a single circRNA, each column indicates a single sample.

Techniques Used: Expressing, Microarray

mRNAs comparing the IRI and sham groups and between the IPC and IRI groups. The left (scatterplots) and right (volcano plots) show the alteration of mRNA expression between IRI and sham groups (A) and between IPC and IRI groups (B) . In the plot, red and green points represent significant altered mRNAs (fold change ≥ 1.5, P -value < 0.05), respectively. (C) Heat maps of mRNA profiles from the microarray data. The color scales represent expression values. Red represents high expression, and green indicates low relative expression. Each row of colored boxes indicates single circRNA; each column indicates single sample.
Figure Legend Snippet: mRNAs comparing the IRI and sham groups and between the IPC and IRI groups. The left (scatterplots) and right (volcano plots) show the alteration of mRNA expression between IRI and sham groups (A) and between IPC and IRI groups (B) . In the plot, red and green points represent significant altered mRNAs (fold change ≥ 1.5, P -value < 0.05), respectively. (C) Heat maps of mRNA profiles from the microarray data. The color scales represent expression values. Red represents high expression, and green indicates low relative expression. Each row of colored boxes indicates single circRNA; each column indicates single sample.

Techniques Used: Expressing, Microarray

Selected circRNA and mRNA qRT-PCR verification between the IRI and sham groups (A) and between the IPC and IRI groups (B) . Data are expressed as the means ± SD, n = 6. * P <0.05 compared with the sham, and ** P <0.01 compared with the sham group; ## P <0.01 compared with the IRI group.
Figure Legend Snippet: Selected circRNA and mRNA qRT-PCR verification between the IRI and sham groups (A) and between the IPC and IRI groups (B) . Data are expressed as the means ± SD, n = 6. * P <0.05 compared with the sham, and ** P <0.01 compared with the sham group; ## P <0.01 compared with the IRI group.

Techniques Used: Quantitative RT-PCR

Identification of circRNAs related to hepatic protection by IPC and qRT-PCR validation. (A) The Venn diagram shows the circRNAs with an opposite direction of the alterations between the two comparison groups (sham vs. IRI and IRI vs. IPC). (B) qRT-PCR detected expression levels of circRNA_017753 in mouse liver among the three groups. Data are expressed as the means ± SD, n = 6. * P <0.05 compared with the sham group; # P <0.05 compared with the IRI group.
Figure Legend Snippet: Identification of circRNAs related to hepatic protection by IPC and qRT-PCR validation. (A) The Venn diagram shows the circRNAs with an opposite direction of the alterations between the two comparison groups (sham vs. IRI and IRI vs. IPC). (B) qRT-PCR detected expression levels of circRNA_017753 in mouse liver among the three groups. Data are expressed as the means ± SD, n = 6. * P <0.05 compared with the sham group; # P <0.05 compared with the IRI group.

Techniques Used: Quantitative RT-PCR, Expressing

The identified circRNAs and its predicted miRNA response elements (MREs).
Figure Legend Snippet: The identified circRNAs and its predicted miRNA response elements (MREs).

Techniques Used:

(A) Prediction of MiRNA and the circRNA–miRNA–mRNA pathway. Detailed structure of circRNA/miRNA interactions. The MRE sequence, miRNA seed type, precise base positions and target miRNA nucleotides are presented as annotation in 2-D structure column. Thirty nucleotides downstream and upstream the seed sequence are presented in “local AU.” Red bars present A/U, and black bars present G/C. The most likely relative MRE position are presented in position column. (B) Overlap of the predicted mRNA target related to circRNA_017753 and significantly upregulated mRNAs caused by IPC intervention.
Figure Legend Snippet: (A) Prediction of MiRNA and the circRNA–miRNA–mRNA pathway. Detailed structure of circRNA/miRNA interactions. The MRE sequence, miRNA seed type, precise base positions and target miRNA nucleotides are presented as annotation in 2-D structure column. Thirty nucleotides downstream and upstream the seed sequence are presented in “local AU.” Red bars present A/U, and black bars present G/C. The most likely relative MRE position are presented in position column. (B) Overlap of the predicted mRNA target related to circRNA_017753 and significantly upregulated mRNAs caused by IPC intervention.

Techniques Used: Sequencing


Figure Legend Snippet:

Techniques Used:


Figure Legend Snippet:

Techniques Used:



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Image Search Results


Primer sequences used for qRT-PCR analysis of  circRNA  and mRNA levels.

Journal: Frontiers in Medicine

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

doi: 10.3389/fmed.2021.626948

Figure Lengend Snippet: Primer sequences used for qRT-PCR analysis of circRNA and mRNA levels.

Article Snippet: Such cRNAs were then hybridized to the Arraystar circRNA array V 2 .0 (8x15K).

Techniques:

CircRNAs comparing the IRI and sham groups and between the IPC and IRI groups. The left (scatterplots) and right (volcano plots) show the alteration of circRNA expression between IRI and sham groups (A) and between IPC and IRI groups (B) . In the scatterplot, the values on the X - and Y -axes are the log2 scaled signals of samples. Fold change is represented by the green lines. The circRNAs outside the range formed by the upper and lower green lines are those with the fold change ≥ 1.5 between the compared groups. In the volcano plot, the vertical green lines represent a 1.5-fold change although the horizontal green line corresponds to a P -value of 0.05. The red points in the volcano plot represent the significantly altered circRNAs with P -value <0.05. (C) Different subgroups of significantly altered circRNAs according to their effects and position. (D) Heat maps of circRNA profiles from the microarray data. The color scales represent expression values. Red represents high expression, and green indicates low relative expression. Each row of colored boxes indicates a single circRNA, each column indicates a single sample.

Journal: Frontiers in Medicine

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

doi: 10.3389/fmed.2021.626948

Figure Lengend Snippet: CircRNAs comparing the IRI and sham groups and between the IPC and IRI groups. The left (scatterplots) and right (volcano plots) show the alteration of circRNA expression between IRI and sham groups (A) and between IPC and IRI groups (B) . In the scatterplot, the values on the X - and Y -axes are the log2 scaled signals of samples. Fold change is represented by the green lines. The circRNAs outside the range formed by the upper and lower green lines are those with the fold change ≥ 1.5 between the compared groups. In the volcano plot, the vertical green lines represent a 1.5-fold change although the horizontal green line corresponds to a P -value of 0.05. The red points in the volcano plot represent the significantly altered circRNAs with P -value <0.05. (C) Different subgroups of significantly altered circRNAs according to their effects and position. (D) Heat maps of circRNA profiles from the microarray data. The color scales represent expression values. Red represents high expression, and green indicates low relative expression. Each row of colored boxes indicates a single circRNA, each column indicates a single sample.

Article Snippet: Such cRNAs were then hybridized to the Arraystar circRNA array V 2 .0 (8x15K).

Techniques: Expressing, Microarray

mRNAs comparing the IRI and sham groups and between the IPC and IRI groups. The left (scatterplots) and right (volcano plots) show the alteration of mRNA expression between IRI and sham groups (A) and between IPC and IRI groups (B) . In the plot, red and green points represent significant altered mRNAs (fold change ≥ 1.5, P -value < 0.05), respectively. (C) Heat maps of mRNA profiles from the microarray data. The color scales represent expression values. Red represents high expression, and green indicates low relative expression. Each row of colored boxes indicates single circRNA; each column indicates single sample.

Journal: Frontiers in Medicine

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

doi: 10.3389/fmed.2021.626948

Figure Lengend Snippet: mRNAs comparing the IRI and sham groups and between the IPC and IRI groups. The left (scatterplots) and right (volcano plots) show the alteration of mRNA expression between IRI and sham groups (A) and between IPC and IRI groups (B) . In the plot, red and green points represent significant altered mRNAs (fold change ≥ 1.5, P -value < 0.05), respectively. (C) Heat maps of mRNA profiles from the microarray data. The color scales represent expression values. Red represents high expression, and green indicates low relative expression. Each row of colored boxes indicates single circRNA; each column indicates single sample.

Article Snippet: Such cRNAs were then hybridized to the Arraystar circRNA array V 2 .0 (8x15K).

Techniques: Expressing, Microarray

Selected circRNA and mRNA qRT-PCR verification between the IRI and sham groups (A) and between the IPC and IRI groups (B) . Data are expressed as the means ± SD, n = 6. * P <0.05 compared with the sham, and ** P <0.01 compared with the sham group; ## P <0.01 compared with the IRI group.

Journal: Frontiers in Medicine

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

doi: 10.3389/fmed.2021.626948

Figure Lengend Snippet: Selected circRNA and mRNA qRT-PCR verification between the IRI and sham groups (A) and between the IPC and IRI groups (B) . Data are expressed as the means ± SD, n = 6. * P <0.05 compared with the sham, and ** P <0.01 compared with the sham group; ## P <0.01 compared with the IRI group.

Article Snippet: Such cRNAs were then hybridized to the Arraystar circRNA array V 2 .0 (8x15K).

Techniques: Quantitative RT-PCR

Identification of circRNAs related to hepatic protection by IPC and qRT-PCR validation. (A) The Venn diagram shows the circRNAs with an opposite direction of the alterations between the two comparison groups (sham vs. IRI and IRI vs. IPC). (B) qRT-PCR detected expression levels of circRNA_017753 in mouse liver among the three groups. Data are expressed as the means ± SD, n = 6. * P <0.05 compared with the sham group; # P <0.05 compared with the IRI group.

Journal: Frontiers in Medicine

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

doi: 10.3389/fmed.2021.626948

Figure Lengend Snippet: Identification of circRNAs related to hepatic protection by IPC and qRT-PCR validation. (A) The Venn diagram shows the circRNAs with an opposite direction of the alterations between the two comparison groups (sham vs. IRI and IRI vs. IPC). (B) qRT-PCR detected expression levels of circRNA_017753 in mouse liver among the three groups. Data are expressed as the means ± SD, n = 6. * P <0.05 compared with the sham group; # P <0.05 compared with the IRI group.

Article Snippet: Such cRNAs were then hybridized to the Arraystar circRNA array V 2 .0 (8x15K).

Techniques: Quantitative RT-PCR, Expressing

The identified circRNAs and its predicted miRNA response elements (MREs).

Journal: Frontiers in Medicine

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

doi: 10.3389/fmed.2021.626948

Figure Lengend Snippet: The identified circRNAs and its predicted miRNA response elements (MREs).

Article Snippet: Such cRNAs were then hybridized to the Arraystar circRNA array V 2 .0 (8x15K).

Techniques:

(A) Prediction of MiRNA and the circRNA–miRNA–mRNA pathway. Detailed structure of circRNA/miRNA interactions. The MRE sequence, miRNA seed type, precise base positions and target miRNA nucleotides are presented as annotation in 2-D structure column. Thirty nucleotides downstream and upstream the seed sequence are presented in “local AU.” Red bars present A/U, and black bars present G/C. The most likely relative MRE position are presented in position column. (B) Overlap of the predicted mRNA target related to circRNA_017753 and significantly upregulated mRNAs caused by IPC intervention.

Journal: Frontiers in Medicine

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

doi: 10.3389/fmed.2021.626948

Figure Lengend Snippet: (A) Prediction of MiRNA and the circRNA–miRNA–mRNA pathway. Detailed structure of circRNA/miRNA interactions. The MRE sequence, miRNA seed type, precise base positions and target miRNA nucleotides are presented as annotation in 2-D structure column. Thirty nucleotides downstream and upstream the seed sequence are presented in “local AU.” Red bars present A/U, and black bars present G/C. The most likely relative MRE position are presented in position column. (B) Overlap of the predicted mRNA target related to circRNA_017753 and significantly upregulated mRNAs caused by IPC intervention.

Article Snippet: Such cRNAs were then hybridized to the Arraystar circRNA array V 2 .0 (8x15K).

Techniques: Sequencing

Journal: Frontiers in Medicine

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

doi: 10.3389/fmed.2021.626948

Figure Lengend Snippet:

Article Snippet: Such cRNAs were then hybridized to the Arraystar circRNA array V 2 .0 (8x15K).

Techniques:

Journal: Frontiers in Medicine

Article Title: Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

doi: 10.3389/fmed.2021.626948

Figure Lengend Snippet:

Article Snippet: Such cRNAs were then hybridized to the Arraystar circRNA array V 2 .0 (8x15K).

Techniques: